Silvia Bruno


M. Sc. in Physics, University of Genoa, Italy.
PhD in Biotechnology, University of Genoa, Italy

Research activity
The main research activity of Silvia Bruno focuses on the elucidation of cell death and cell proliferation mechanisms, with particular regard to hematologic malignancies.

2017 -2019 : Assistant Professor (RTDB) at the Department of Experimental Medicine, University of Genova, Italy. Gets hold of the National Academic Qualification as Associate Professor in ‘Human Anatomy’ and ‘Applied Biology’. Studies on targeting cell metabolism in chronic lymphocytic leukemia.
1998 - 2016: Research Associate at the Department of Experimental Medicine, University of Genova, Italy. Studies on “Structure of immunoglobulins in chronic lymphocytic leukemia”, “Modulation of apoptotic mechanisms in hematologic malignancies by novel molecules”, “Role of PML gene on immune evasion of tumor cells”, “Role of CD44 in squamous cell carcinogenesis”, ”Role of CD85j/ILT2 molecular interaction in immune control of viral infection”. AIRC, PRIN and Fondazione San Paolo grants, to Carlo E. Grossi, Ermanno Ciccone and Franco Fais
1993 - 1997: Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy. Research Fellow, “Cell cycle and apoptosis effects of fenretinide on bladder cancer by analytical cytometry”. NIH-NCI (National Institute of Health/National Cancer Institute, USA) grant, PI Andrea Decensi.
1990-1992: Appointed ‘Research Assistant Professor of Medicine’ by the New York Medical College (NYMC), Valhalla, NY (Cancer Research Institute). “Advanced in situ flow and image cytometry for studies on cell proliferation and cell death”. (NIH-NCI grant, PI Zbigniew Darzynkiewicz).
1989 - 1990: Memorial Sloan Kettering Cancer Center, New York, NY (Visiting scientist, Zbigniew Darzynkiewicz lab, NIH-NCI grant on ‘New antitumor drugs’).
1986 - 1989: Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy. “Analytical cytometry for cell cycle and tumor progression studies”. (AIRC Research contracts).
1985: Biophysics and Radiation Biology Institute, University of Hamburg, Germany. “Flow cytometry for cell cycle analysis”. (Visiting fellow, Heinz Baisch lab).
1984 - 1985: Physics Department, University of Perugia, Italy. Research assistant. “Non-Linear Electron Spin Resonance for studies on bipolar lipid dynamics”. (CNR fellowship).

Silvia Bruno has published more more than 70 peer-reviewed papers (total H-index n=28, total citations more than 4000), 6 book chapters and invited reviews.
Has been invited in international symposia as Lecturer and Keynote Lecturer.
Is member of the International Society for Advanced Cytometry (ISAC).
Performs reviewing activity for scientific journals (Cytometry, Journal of Immunology, Leukemia, Oncotarget, etc.)
Lecturer at the Medical and Pharmaceutical School of the University of Genoa for the Human Anatomy program.

Selected publications
Bruno S, Ledda B, Tenca C, Ravera S, Orengo AM, Mazzarello AN, Pesenti E, Casciaro S, Racchi O, Ghiotto F, Marini C, Sambuceti G, DeCensi A, Fais F.
Metformin inhibits cell cycle progression of B-cell chronic lymphocytic leukemia cells.
Oncotarget. 2015 6:22624-40.

Cossu I, Bottoni G, Loi M, Emionite L, Bartolini A, Di Paolo D, Brignole C, Piaggio F, Perri P, Sacchi A, Curnis F, Gagliani MC, Bruno S, Marini C, Gori A, Longhi R, Murgia D, Sementa AR, Cilli M, Tacchetti C, Corti A, Sambuceti G, Marchiò S, Ponzoni M, Pastorino F.
Neuroblastoma-targeted nanocarriers improve drug delivery and penetration, delay tumor growth and abrogate metastatic diffusion.
Biomaterials. 2015 68:89-99.

Marcatili P, Ghiotto F, Tenca C, Chailyan A, Mazzarello AN, Yan XJ, Colombo M, Albesiano E, Bagnara D, Cutrona G, Morabito F, Bruno S, Ferrarini M, Chiorazzi N, Tramontano A, Fais F. Igs expressed by chronic lymphocytic leukemia B cells show limited binding-site structure variability. J Immunol. 2013, 190:5771-8.

Bruno S, Ghiotto F, Tenca C, Mazzarello A, Bono M, Luzzi P, Casciaro S, Recchia A, DeCensi A, Morabito F and Fais F. N-(4-hydroxyphenyl)retinamide promotes apoptosis of resting and proliferating B-cell chronic lymphocytic leukemia cells and potentiates Fludarabine and ABT-737 cytotoxicity. Leukemia 2012 26:2260-8.

Ghiotto F, Fais F, Bruno S. BH3-only proteins: the death-puppeteer's wires. Cytometry 2010 77:11-21.

Bruno S, Ghiotto F, Fais F, Fagioli M, Luzi L, Pelicci PG, Grossi CE, Ciccone E. The PML gene is not involved in the regulation of MHC class I expression in human cell lines. Blood. 2003 101:3514-19.

Bruno S, Tenca C, Saverino D, Ciccone E, Grossi CE. Apoptosis of squamous cells at different stages of carcinogenesis following 4-HPR treatment. Carcinogenesis 2002 23:447-56

Bruno S, Fabbi M, Tiso M, Santamaria B, Ghiotto F, Saverino D, Tenca C, Zarcone D, Ferrini S, Ciccone E, Grossi CE. Cell activation via CD44 occurs in advanced stages of squamous cell carcinogenesis. Carcinogenesis. 2000 21:893-900.

Decensi A, Fontana V, Bruno S, Gustavino C, Gatteschi B, Costa A. Effect of tamoxifen on endometrial proliferation. J Clin Oncol. 1996 14:434-40.

Bruno S, Ardelt B, Skierski JS, Traganos F, Darzynkiewicz Z. Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes. Cancer Res. 1992 52:470-3.

Bruno S, Del Bino G, Lassota P, Giaretti W, Darzynkiewicz Z. Inhibitors of proteases prevent endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Leukemia. 1992 6:1113-20.